Oct. 30, 2012, 11:24 PM
#4
Thank you. The whole reason I decided to ask is because there was a website that said they offered sexing for semen that had been frozen before. Granted, it was for bulls, but they mentioned reverse sorting. I know when I was in college we mostly worked with bull semen with maybe some boar semen as well, so I can't speak to why this wouldn't work with equines. Guess I should go rummage in some boxes and find my old repro notes. I'll send you a PM
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Oct. 31, 2012, 06:45 AM
#5
'Sex-sorted' bull semen, which we use successfully, is centrifuged (spun quickly), leveraging the weight difference between male and female chromosomes in the semen. It is not a perfect science, in that it gives a 90% chance of accurately determining sex.
The process also measurably reduces efficacy and fertility of the semen.
Cattle repro is much more robust than equine, and also much further advanced in its R&D. Everyone needs to eat. Not everyone needs a horse.
Advances have occurred in equine repro, such as ICSI and the ability to freeze, thaw, then successfully implant embryos. To date, however, my understanding is that there is no commercially proven equine equivalent to this bovine practice. Furthermore, you seem to want to sex-select from existing frozen. This would be much more fickle again, as the more you mess with the process, the lower the fertility becomes.
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